nafise sadeghi
5th June 2009, 12:08 AM
ANALYSIS OF GLOMALIN-mycorrhizal fungi in soil and in roots: (http://alonefarmer.blogfa.com/post-1495.aspx)
Glomalin is a glycoprotein produced abundantly on hyphae and spores of
arbuscular mycorrhizal fungi in soil and in roots. Shortly after we Sara Wright and I collaborated on development of a monoclonal antibody specific for Paraglomus occultum, we attempted to produce monoclonal antibodies against other AM fungi. With more recent species (e.g. Glomus intraradices), antibody cell lines were broader in specificity but restricted to Glomeromycota. Using these cell lines, Sara Wright and co-workers discovered they reacted specifically against glomalin. These antibodies then became a tool to detect and quantify glomalin associated with fungal biomass and in a wide range of soils
http://invam.caf.wvu.edu/methods/mycorrhizae/aggregate_s.jpg (http://invam.caf.wvu.edu/methods/mycorrhizae/aggregate.jpg)http://invam.caf.wvu.edu/methods/mycorrhizae/morrowiae_s.jpg (http://invam.caf.wvu.edu/methods/mycorrhizae/morrowiae.jpg)
Glomalin is a glycoprotein produced abundantly on hyphae and spores of
arbuscular mycorrhizal fungi in soil and in roots. Shortly after we Sara Wright and I collaborated on development of a monoclonal antibody specific for Paraglomus occultum, we attempted to produce monoclonal antibodies against other AM fungi. With more recent species (e.g. Glomus intraradices), antibody cell lines were broader in specificity but restricted to Glomeromycota. Using these cell lines, Sara Wright and co-workers discovered they reacted specifically against glomalin. These antibodies then became a tool to detect and quantify glomalin associated with fungal biomass and in a wide range of soils
http://invam.caf.wvu.edu/methods/mycorrhizae/aggregate_s.jpg (http://invam.caf.wvu.edu/methods/mycorrhizae/aggregate.jpg)http://invam.caf.wvu.edu/methods/mycorrhizae/morrowiae_s.jpg (http://invam.caf.wvu.edu/methods/mycorrhizae/morrowiae.jpg)